brucellosis is an infectious disease and it seems that it affects human immune system and can cause acute, subacute and chronic clinical features. forty patients suffering from chronic brucellosis were studied for cd3+, cd4+,cd8+, cd19+, cells and cd4+/cd8+ ratio by flow cytometry before and after treatment with antibiotics and immunopotentiators. the results were compared with 15 healthy contr...

background programmed cell death (apoptosis) is a physiological process needed to remove unwanted or damaged cells. it has been hypothesized that any failure of programmed cell death leads to the development of neoplasm. identifying new agents which induce apoptosis in tumor cells is of great significance in treatment of neo­plasms. numerous studies suggest that exposure of tumor cells to stati...

background sulfur oxides released from the burning of oil causes severe environmental pollution. the sulfur can be removed via the 4s pathway in biodesulfurization (bds). immobilization approaches have been developed to prevent cell contamination of oil during the bds process. objectives the encapsulation of rhodococcus erythropolis r1 in calcium alginate beads was studied in order to enhance c...

background: magnetic nanoparticles in a variable magnetic field are able to produce heat. this heat (42-45°c) has more selective effect on fast dividing cancer cells than normal tissues. methods: in this work magnetite nanoparticles have been prepared via co-precipitation and phase identification was performed by powder x-ray diffraction (xrd). magnetic parameters of the prepared nanoparticles ...

intraplatelet phospho-vasp analysis is a commonly used approach for monitoring of anti-platelet therapy; however, testing of intraplatelet phospho-vasp expression needs a high level of experience and proficiency. the ability to recognize how the pre-analytical variations can change the results would be helpful for the interpretation of data from intraplatelet p-vasp analysis. the aim of this st...

background :   chitosan is unbranched polysaccharide composed of d-glucosamine and n-acetyl-d-glucosamine. chitosan, derived from shrimp shell, has broad antimicrobial properties against gram-negative, gram-positive bacteria and fungi.   methods :  chitosan was extracted from shrimp shell and studied for cell entrapment and anti-bacterial properties. the hydrogel chitosan was used as the beads ...

conclusions this study delineated the beneficial role of rsv for growth regulation of skin cancer cells and highlighted the involvement of rsv in the induction of apoptosis in a431 cells. these findings might conduct evidence into the oncolytic properties of rsv in the skin cancer. further studies are required to indicate intracellular targets for rsv-induced apoptosis in skin cancer cells. res...

introduction: acute leukemia is the most common malignancy in children and acute lymphoblastic leukemia (all) accounts for 75% of acute leukemia cases. new treatment protocols have resulted in complete remission rates up to nearly 100% in children with acute lymphoblastic leukemia. today, one of the most important prognostic factors in acute lymphoblastic leukemia is intensity of the treatment....

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

background: differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management. objective: we analyzed the nuclear dna content of various types of hydropic placentas by flowcytometry. materials and methods: dna ploidy analysis was performed i...